[1]王 娟,王 薇.“森淼”文冠果新品系组织培养技术[J].陕西林业科技,2018,(03):7-10+14.
 WANG Juan,WAMG Wei.Tissue Culture of Xanthoceras sorbifolia “Senmiao”[J].,2018,(03):7-10+14.
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“森淼”文冠果新品系组织培养技术()
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《陕西林业科技》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
7-10+14
栏目:
试验与调查研究
出版日期:
2018-06-25

文章信息/Info

Title:
Tissue Culture of Xanthoceras sorbifolia “Senmiao”
文章编号:
1001-2117(2018)03-0007-04
作者:
王 娟1王 薇2
1.陕西省林业国际合作项目管理中心; 2.陕西省林业教育培训站,西安 710082
Author(s):
WANG Juan1WAMG Wei2
1.International Forestry Cooperation Program Administration Center, Forestry Department of Shaanxi, Xi'an, Shaanxi 710082; 2.Shaanxi Forestry Education and training Station, Xi'an, Shaanxi 710082
关键词:
文冠果 休眠枝 水培 组织培养
Keywords:
Xanthoceras sorbifolia hydroponics tissue culture
分类号:
S723.1+32.6
文献标志码:
A
摘要:
以文冠果新品系“森淼”休眠枝水培嫩枝为外植体,研究了消毒时间对启动培养、植物生长调节剂对增殖培养与生根培养的影响,以建立一种启动培养污染率低、增殖系数与生根率相对较高的“森淼”新品系组织培养体系。结果表明:以休眠枝水培嫩枝为外植体可从源头控制启动培养的污染率,0.1%升汞消毒8 min可将污染率控制在10%以内; 在MS附加1.0 mg·L-16-BA以及0.5 mg·L-1 IBA的培养基中外植体腋芽萌发快,萌发率高; MS附加2.0 mg·L-1 6-BA以及0.5 mg·L-1 IBA的培养基适合“森淼”品系无菌芽增殖,平均每个外植体增殖芽数为3.7; 1/2MS附加0.5 mg·L-1 IBA的培养基适合“森淼”品系无菌芽生根,生根率可达91.4%,NAA较IBA的生根效果差。
Abstract:
Effects of sterilization time on preliminary culture and plant growth regulators on enrichment and rooting culture were studied using fresh branches from dormant branches of Xanthoceras sorbifolia “Senmiao”, a new variety, as explants.The tissue culture system with low contamination rate in preliminary culture stage and high proliferation and rooting rate was established.The result showed that fresh branches from dormant branches of Xanthoceras sorbifolia as explants can control contamination effectively at their source.Contamination rate can be controlled within 10% through 8 minutes of disinfection with 0.1% HgCl2.Axillary buds can germinate rapidly on the MS medium with 1.0 mg·L-1 6-BA and 0.5 mg/L IBA.MS medium with 2.0 mg/L 6-BA and 0.5 mg·L-1 IBA is suitable for enrichment culture of germ-free shoots and average shoot number from each explant is 3.7 on this medium.1/2 MS medium with 0.5 mg/LIBA is suitable for rooting culture of germ-free shoots.The rooting rate is up to 91.4% on this medium.Rooting effect of IBA precede that of NAA.The results will promote rapid propagation and molecular breeding of Xanthoceras sorbifolia “Senmiao”, a oilseed variety of trees.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-03-07
作者简介:王 娟(1981-),女,陕西清涧人,工程师,主要从事国际林业项目管理工作。
更新日期/Last Update: 2018-06-25